Publications & Patents

原著論文

• Taguchi T, Yokoyama M, Fujita T, Tanba S, Oikiri H, Osawa Y, Matsumura Y, Shigeto T, Yokoyama Y, Fujii H: Utility of circulating human papillomavirus DNA as a biomarker for detection and prognosis of cervical cancer in Japanese patients. Int. J. Clin. Oncol. (2025) doi: 10.1007/s10147-025-02762-w
  Int. J. Clin. Oncol.
• Tabe C, Fujita T, Taima K, Tanaka H, Makiguchi T, Itoga M, Ishioka Y, Tasaka S, Fujii H: Highly sensitive and accurate detection of ALK-TKI resistance mutations by oligoribonucleotide interference-PCR (ORNi-PCR)-based methods. Lung Cancer (2024) 197, 107969.
  Lung Cancer
• Fujita H, Fujita T, Ishido K, Hakamada K, Fujii H: Oligoribonucleotide interference-PCR-based methods for the sensitive and accurate detection of KRAS mutations. Biol. Methods Protoc. (2024) 9, bpae071.
  Biol. Methods Protoc.
• Kadonosawa Y, Yokoyama M, Tatara Y, Fujita T, Yokoyama Y: Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway. Oncol. Lett. (2024) 28, 359.
  Oncol. Lett.
• Fujita T, Fujii H: iChIP-SILAC analysis identifies epigenetic regulators of CpG methylation of the p16INK4A gene. FEBS Lett. (2024) 598, 1094-1109.
  FEBS Lett.
• Kadonosawa Y, Yokoyama M, Tatara Y, Miyoshi I, Araki Y, Yokoyama Y, Fujii H: Moderate transgenic expression of CBR1 is not sufficient to prolong life expectancy in an SV40 T-antigen-induced gynecological carcinogenesis mouse model. Hirosaki Med. J. (2024) 74, 99-107.
  Hirosaki Med. J.
• Ishidoya M, Fujita T, Tasaka S, Fujii H: Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status. Anal. Chim. Acta (2024) 1302, 342486.
  Anal. Chim. Acta
• Zhang X, Sharma P, Maschmeyer P, Hu Y, Lou M, Kim J, Fujii H, Unutmaz D, Schwabe RF, Winau F: GARP on hepatic stellate cells is essential for the development of liver fibrosis. J. Hepatol. (2023) 79, 1214-1225.
  PubMed
• Fujita T, Nagata S, Fujii H: Oligoribonucleotide-mediated blockade of DNA extension by Taq DNA polymerases increases specificity and sensitivity for detecting single-nucleotide differences. Anal. Chem. (2023) 95, 3442-3451.
  Anal. Chem.
• Yokoyama M, Fujita T, Kadonosawa Y, Tatara Y, Motooka D, Ikawa M, Fujii H, Yokoayama Y: Development of transgenic mice overexpressing mouse carbonyl reductase 1. Mol. Biol. Rep. (2022) 50, 531-540.
  PubMed
• Fujii H, Fujita T: An enChIP system for the analysis of genome functions in budding yeast. Biol. Methods Protoc. (2022) 7, bpac025.
  Biol. Methods Protoc.
• Imanishi S, Nagata S, Fujita T, Fujii H: Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma. Int. J. Exp. Pathol. (2022) 103, 245-251.
  Int. J. Exp. Pathol.
• Fujita H, Fujita T, Fujii H: IL-3-induced immediate expression of c-fos and c-jun is modulated by the IKK2-JNK axis. Cells (2022) 11, 1451.
  Cells
• Sarudate A, Fujita T, Nakayama T, Fujii H: enChIP-Seq analyzer: a software program to analyze and interpret enChIP-Seq data for the detection of physical interactions between genomic regions. Genes (2022) 13, 472.
  Genes
• Fujii H: pSIR-bsr, a self-inactivating retrovirus vector expressing the blasticidin S-resistance gene. Biol. Methods Protoc. (2021) 6, bpab022.
  Biol. Methods Protoc.
• Takeda T, Yokoyama Y, Takahashi H, Okuzaki D, Asai K, Itakura H, Miyoshi N, Kobayashi S, Uemura M, Fujita T, Ueno H, Mori M, Doki Y, Fujii H, Eguchi H, Yamamoto H: A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells. Br. J. Cancer (2021) 126, 109-119.
  Br. J. Cancer
• Fujita T, Nagata S, Fujii H: Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations. Commun. Biol. (2021) 4, 988.
  Commun. Biol.
• Yuno M, Nagata S, Fujita T, Fujii H: MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis. Biol. Methods Protoc. (2021) 6, bpab013.
  Biol. Methods Protoc.
• Fujita T, Nagata S, Yuno M, Fujii H: Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes in vitro. Biol. Methods Protoc. (2021) 6, bpab009.
  Biol. Methods Protoc.
• Shimizu T, Fujita T, Fukushi S, Horino Y, Fujii H: Discrimination of CpG methylation status and nucleotide differences in tissue specimen DNA by oligoribonucleotide interference-PCR. Int. J. Mol. Sci. (2020) 21, 5119.
  Int. J. Mol. Sci.
• Husain A, Xu J, Fujii H, Nakata M, Kobayashi M, Wang JY, Rehwinkel J, Honjo T, Begum NA: SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination. EMBO J. (2020) 39, e102931.
  EMBO J.
• Nasrallah R, Imianowski CJ, Bossini-Castillo L, Grant FM, Dogan M, Placek L, Kozhaya L, Kuo P, Sadiyah F, Whiteside SK, Mumbach MR, Glinos D, Vardaka P, Whyte CE, Lozano T, Fujita T, Fujii H, Liston A, Andrews S, Cozzani A, Yang J, Mitra S, Lugli E, Chang HY, Unutmaz D, Trynka G, Roychoudhuri R: A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by T reg cells. Nature (2020) 583, 447-452.
  Nature
• Baba K, Fujita T, Tasaka S, Fujii H: Simultaneous detection of the T790M and L858R mutations in the EGFR gene by oligoribonucleotide interference-PCR. Int. J. Mol. Sci. (2019) 20, 4020.
  Int. J. Mol. Sci.
• Fujita T, Motooka D, Fujii H: Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR). Biol. Methods Protoc. (2019) 4, bpz009.
  Biol. Methods Protoc.
• Kitaura F, Yuno M, Fujita T, Wakana S, Ueda J, Yamagata K, Fujii H: Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice. PLoS One (2019) 14, e0220199.
  PLoS One
• Fujita T, Yuno M, Kitaura F, Fujii H: A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences. Sci. Rep. (2018) 8, 17195.
  Sci. Rep.
• Fujita T, Yuno M, Fujii H: An enChIP system for the analysis of bacterial genome functions. BMC Res. Notes (2018) 11, 387.
  BMC Res. Notes
• Fujita T, Yuno M, Kitaura F, Fujii H: Detection of genome-edited cells by oligoribonucleotide interference-PCR. DNA Res. (2018) 25, 395-407.
  DNA Res.
• Hamidian A, Vaapil M, von Stedingk K, Fujita T, Persson CU, Eriksson P, Veerla S, De Preter K, Speleman F, Fujii H, Påhlman S, Mohlin S: Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator. Biochem. Biophys. Res. Commun. (2018) 499, 291-298.
  PubMed
• Fujita T, Yuno M, Fujii H: enChIP systems using different CRISPR orthologues and epitope tags. BMC Res. Notes (2018) 11, 154.
  BMC Res. Notes
• Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H: Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Genes Cells (2018) 23, 318-325.
  Genes Cells
• Fujita T, Kitaura F, Yuno M, Suzuki Y, Sugano S, Fujii H: Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line. DNA Res. (2017) 24, 537–548.
  DNA Res.
• Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H: Identification of physical interactions between genomic regions by enChIP-Seq. Genes Cells (2017) 22, 506-520.
  Genes Cells
• Vermeersch E, Denorme F, Maes W, De Meyer SF, Vanhoorelbeke K, Edwards J, Shevach EM, Unutmaz D, Fujii H, Deckmyn H, Tersteeg C: The role of platelet and endothelial GARP in thrombosis and hemostasis. PLoS One (2017) 12, e0173329.
  PLoS One
• Fujita T, Yuno M, Fujii H: Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus. Sci. Rep. (2016) 6, 30485.
  Sci. Rep.
• Fujita T, Yuno M, Fujii H: Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. Genes Cells (2016) 21, 370-377.
  Genes Cells
• Fujita T, Yuno M, Okuzaki D, Ohki R, Fujii H: Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing. PLoS One (2015) 10, e0123387.
  PLoS One
• Fujita T, Kitaura F, and Fujii H: A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells. PLoS One (2015) 10, e0116579.
  PLoS One
• Fujita T, and Fujii H: Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins. BMC Mol. Biol. (2014) 15, 26.
  BMC Mol. Biol.
• Tanigawa N, Fujita T, and Fujii H: Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR): a simple method for suppressing PCR amplification of specific DNA sequences using ORNs. PLoS One (2014) 9, e113345.
  PLoS One
• Fujita T, and Fujii H: Identification of proteins associated with an IFNγ-responsive promoter by a retroviral expression system for enChIP using CRISPR. PLoS One (2014) 9, e103084.
  PLoS One
• Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, and Fujii H: Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci. Rep. (2013) 3, 3171.
  Sci. Rep.
• Fujita T, and Fujii H: Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem. Biophys. Res. Commun. (2013) 439, 132-136.
  Biochem. Biophys. Res. Commun.
• Edwards JP, Fujii H, Zhou AX, Creemers J, Unutmaz D, Shevach EM: Regulation of the Expression of GARP/Latent TGF-β1 Complexes on Mouse T Cells and Their Role in Regulatory T Cell and Th17 Differentiation. J. Immunol. (2013) 190, 5506-5515.
  PubMed
• Zhou ZX, Kozhaya L, Fujii H, and Unutmaz D: GARP-TGF-β complexes negatively regulate regulatory T cell development and maintenance of peripheral CD4+ T cells in vivo. J. Immunol. (2013) 190, 5057-5064.
  PubMed
• Okamoto A, Torigata K, Sakurai MA, Okuzaki D, Fujii H, Ohmine T, Miura D, Kimura S, Yabuta N, and Nojima H: A simple and efficient method for the preparation of live leukocytes from peripheral blood using the LeukoCatchTM system. Adv. Biosci. Biotechnol. (2012) 3, 630-642.
  Adv. Biosci. Biotechnol.
• Fujita T, and Fujii H: Efficient isolation of specific genomic regions by insertional chromatin immunoprecipitation (iChIP) with a second-generation tagged LexA DNA-binding domain. Adv. Biosci. Biotechnol. (2012) 3, 626-629.
  Adv. Biosci. Biotechnol.
• Hutchins AP, Poulain S, Fujii H, and Miranda-Saavedra D: Discovery of new transcripts from RNA-seq data in mouse CD4+ T cells. Genomics (2012) 100, 303-313.
  PubMed
• Fujita T, and Fujii H: Transcription start sites and usage of the first exon of mouse Foxp3 gene. Mol. Biol. Rep. (2012) 39, 9613-9619.
  PubMed
• Juliana FM, Nara H, Onoda T, Rahman M, Araki A, Jin L, Fujii H, Tanaka N, Hoshino T, and Asao H: Apurinic/apyrimidinic endonuclease1/redox factor-1 (Ape1/Ref-1) is essential for IL-21-induced signal transduction through ERK1/2 pathway. Biochem. Biophys. Res. Commun. (2012) 420, 628-634.
  PubMed
• Fujita T, and Fujii H: Direct identification of insulator components by insertional chromatin immunoprecipitation. PLoS One (2011) 6, e26109.
  PLoS One
• Fujita T, and Fujii H: Species-specific 5'-genomic structure and multiple transcription start sites in the chicken Pax5 gene. Gene (2011), 477, 24-31.
  PubMed
• Hoshino A, and Fujii H: Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions. J. Biosci. Bioeng. (2009), 108, 446-449.
  PubMed
• Fujii H: Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene. J. Biosci. Bioeng. (2009), 108, 438-40.
  PubMed
• Saint Fleur S, Hoshino A, Kondo K, Egawa T, and Fujii H: Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon. Blood (2009), 114, 1355-1365.
  PubMed
• Wang R, Kozhaya L, Khaitan A, Fujii H, and Unutmaz: Expression of GARP selectively identifies activated human Foxp3+ regulatory T cells. Proc. Natl. Acad. Sci. USA (2009), 106, 13439-44.
  PubMed
• Fujii H, and Hoshino A: Lack of nuclear translocation of cytoplasmic domains of IL-2/IL-15 receptor subunits. Cytokine (2009) 46, 302-308.
  PubMed
• Wang R, Wan Q, Kozhaya L, Fujii H, and Unutmaz D: Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression. PLoS One (2008) 3, e2705 doi:10.1371/journal.pone.0002705
  PubMed
• Fujii H: Receptor expression is essential for proliferation induced by dimerized Jak kinases. Biochem. Biophys. Res. Commun. (2008) 370, 557-560.
  PubMed
• Singh AP, Buscaglia CA, Wang Q, Levay A, Nussenzweig DR, Walker J, Winzeler EA, Fujii H, Fonoura BMA, and Nussenzweig V: Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite. Cell (2007) 131, 492-504.
  PubMed
• Hoshino A, and Fujii H: Temporal regulation of Stat5 activity determines cell differentiation program. Biochem. Biophys. Res. Commun. (2007) 358, 914-919.
  PubMed
• Hoshino A, Hirst JA, and Fujii H: Reglation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase. J. Biol. Chem. (2007) 282, 17706-17711.
  PubMed
• Hoshino A, and Fujii H: Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress. Cell Stress Chaperones (2007) 12, 186-191.
  PubMed
• Hoshino A, and Fujii H: Redundant promoter elements mediate IL-3-induced expression of a novel cytokine-inducible gene, cyclon. FEBS Lett. (2007) 581, 975-980.
  PubMed
• Fujii H: Cell type-specific roles of Jak3 in IL-2-induced proliferative signal transduction. Biochem. Biophys. Res. Commun. (2007) 354, 825-829.
  PubMed
• Hoshino A, Saint Fleur S, and Fujii H: Regulation of Stat1 protein expression by phenylalanine 172 in the coiled-coil domain. Biochem. Biophys. Res. Commun. (2006) 346, 1062-1066.
  PubMed
• Hoshino A, Matsumura S, Kondo K, Hirst JA, and Fujii H: Inducible translocation trap: a system to detect translocation of signaling molecules induced by extracellular stimuli. Mol. Cell (2004) 15, 153-159.
  PubMed
• Tucker SN, Jessup HK, Fujii H, and Wilson CB: Enforced expression of the Ikaros isoform IK5 decreases the numbers of extrathymic IEL and NK1.1+ T cells. Blood (2002) 99, 513-519.
  PubMed
• Gilmour KC, Fujii H, Cranston T, Davies EG, Kinnon C, and Gaspar HB: Defective expression of the interleukin-2/interleukin-15 receptor β subunit leads to a natural killer cell-deficient form of severe combined immunodeficiency. Blood (2001) 98, 877-879.
  PubMed
• Tsujino S, Di Santo JD, Takaoka A, McKernan TL, Noguchi S, Taya C, Yonekawa H, Saito T, Taniguchi T, and Fujii H: Differential requirement of the cytoplasmic subregions of γc in T cell development and function. Proc. Natl. Acad. Sci. USA (2000) 97, 10514-10519.
  PubMed
• Tsujino S, Miyazaki T, Kawahara A, Maeda M, Taniguchi T, and Fujii H: Critical role of the membrane-proximal, proline-rich motif of the interleukin-2 receptor γc chain in the Jak3-independent signal transduction. Genes Cells (1999) 4, 363-373.
  PubMed
• Takaoka A, Tanaka N, Mitani Y, Miyazaki T, Fujii H, Sato M, Kovarik P, Decker T, Schlessinger J, and Taniguchi T: Protein tyrosine kinase Pyk2 mediates the Jak-dependent activation of MAPK and Stat1 in IFNγ, but not IFNα, signaling. EMBO J. (1999) 18, 2480-2488.
  PubMed
• Fujii H, Ogasawara K, Otsuka H, Suzuki M, Yamamura K-i, Yokochi T, Miyazaki T, Suzuki H, Mak TW, Taki S, and Taniguchi T: Functional dissection of the cytoplasmic subregions of the IL-2 receptor βc chain in primary lymphocyte populations. EMBO J. (1998) 17, 6551-6557.
  PubMed
• Miyazaki T, Takaoka A, Nogueira L, Dikic I, Fujii H, Tsujino S, Mitani Y, Maeda M, Schlessinger J, and Taniguchi T: Pyk2 is a downstream mediator of the IL-2 receptor-coupled Jak signaling pathway. Genes Dev. (1998) 12, 770-775.
  PubMed
• Fujii H, Nakagawa Y, Schindler U, Kawahara A, Mori H, Gouilleux F, Groner B, Ihle JN, Minami Y, Miyazaki T, and Taniguchi T: Activation of Stat5 by interleukin 2 requires a carboxyl-terminal region of the interleukin 2 receptor β chain but is not essential for the proliferative signal transmission. Proc. Natl. Acad. Sci. USA (1995) 92, 5482-5486.
  PubMed
• Miyazaki T, Kawahara A, Fujii H, Nakagawa Y, Minami Y, Liu Z-J, Oishi I, Silvennoinen O, Witthuhn BA, Ihle JN, and Taniguchi T: Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science (1994) 266, 1045-1047.
  PubMed
• Tsutsumi A, Kubo M, Fujii H, Freire-Moar J, Turck CW, and Ransom JT: Regulation of protein kinase C isoform proteins in phorbol ester-stimulated Jurkat T lymphoma cells. J. Immunol. (1993) 150, 1746-1754.
  PubMed

総説

• Ishidoya M, Fujita T, and Fujii H: Blocking RPA-based methods for the determination of CpG methylation status and detection of gene mutations. Epigenomics (2024) 17, 141-143.
  Epigenomics
• Shimizu T, Fujita T, and Fujii H: Oligoribonucleotide interference-PCR: principles and applications. Biol. Methods Protoc. (2022) 7 (1), bpac010.
  Biol. Methods Protoc.
• Fujita H, Fujita T, and Fujii H: Locus-specific genomic DNA purification using the CRISPR system: methods and applications. CRISPR J. (2021) 4 (2), 290-300.
  CRISPR J.
• Fujita T, and Fujii H: Purification of specific DNA species using the CRISPR system. Biol. Methods Protoc. (2019) 4 (1), bpz008.
  Biol. Methods Protoc.
• 藤井　穂高：遺伝子座特異的クロマチン免疫沈降法によるゲノム機能発現調節機構の生化学的解析. 弘前医学 (2019) 69 (1-4): 10-18.
  弘前医学
• Fujita T, and Fujii H: in vitro engineered DNA-binding molecule-mediated chromatin immunoprecipitation (in vitro enChIP) using CRISPR ribonucleoproteins in combination with next-generation sequencing (in vitro enChIP-Seq) for the identification of chromosomal interactions. Bio-protocol (2017) 7 (22), doi: 10.21769/BioProtoc.2612.
  Bio Protoc.
• 藤井　穂高、藤田敏次：遺伝子座特異的クロマチン免疫沈降法による特定ゲノム領域結合分子の同定とゲノム高次構造解析. 生体の科学（医学書院）(2017) 6月号、68 (3): 189-193.
• Fujita T, and Fujii H: Isolation of specific genomic regions and identification of associated molecules by enChIP. JoVE (2016) issue 107, doi: 10.3791/53478.
  JoVE
• Fujita T, and Fujii H: Biochemical analysis of genome functions using locus-specific chromatin immunoprecipitation technologies. Gene Regul. Syst. Bio. (2016) Suppl. 1, 1-9.
  Gene Regul. Syst. Bio.
• 藤井　穂高: 遺伝子座特異的クロマチン免疫沈降法によるエピジェネティック創薬. 化学工業（化学工業社）(2015) 12月号、66 (12):28-34.
• Fujita T, and Fujii H: Applications of engineered DNA-binding molecules such as TAL proteins and the CRISPR/Cas system in biology research. Int. J. Mol. Sci. (2015) 16, 23143-23164.
  Int. J. Mol. Sci.
• Fujii H, and Fujita T: Isolation of specific genomic regions and identification of their associated molecules by engineered DNA-Binding molecule-mediated chromatin immunoprecipitation (enChIP) using the CRISPR system and TAL proteins. Int. J. Mol. Sci. (2015) 16, 21802-21812.
  Int. J. Mol. Sci.
• 藤田　敏次、藤井　穂高: CRISPR/Casのゲノム編集以外への応用. 実験医学（羊土社）(2014) 7月号、32 (11):1732-1736.
• Fujita T, and Fujii H: Identification of proteins interacting with genomic regions of interest in vivo using engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Bio-protocol (2014) 4 (10), doi: 10.21769/BioProtoc.1124.
  Bio Protoc.
• 藤田　敏次、藤井　穂高: 挿入的クロマチン免疫沈降法 (iChIP) による特定ゲノム領域結合分子の網羅的同定. 実験医学（羊土社）(2013) 10月号、31 (16):2629-2636.
• Fujita T, and Fujii H: Locus-specific biochemical epigenetics/chromatin biochemistry by insertional chromatin immunoprecipitation. ISRN Biochem. (2013) 2013, 913273.
  ISRN Biochem.
• Saint Fleur S, and Fujii H: Cytokine-induced nuclear translocation of signaling proteins and their analysis using the inducible translocation trap system. Cytokine (2008) 43, 187-197.
  PubMed
• Fujii H: Mechanisms of signal transduction from receptors of Type I and Type II cytokines. J. Immunotoxicol. (2007) 4, 69-76.
  PubMed
• Taniguchi T, Miyazaki T, Minami Y, Kawahara A, Fujii H, Nakagawa Y, Hatakeyama M, and Liu Z-J: IL-2 signaling involves recruitment and activation of multiple protein tyrosine kinases by the IL-2 receptor. Ann. N. Y. Acad. Sci. (1995) 766, 235 - 244.
  PubMed

著書

• Fujita T, and Fujii H: New directions for epigenetics: application of engineered DNA-binding molecules to locus-specific epigenetic research. Handbook of Epigenetics, Third Edition, Academic Press (Elsevier), Editor: Trygve O. Tollefsbol, (2022) 843-868.
• 藤田　敏次、藤井　穂高：CRISPRを利用した配列特異的なDNAの単離　実験医学別冊「完全版　ゲノム編集実験スタンダード」（羊土社）　編集：山本　卓，佐久間　哲史　(2019) 309-316.
• 藤田　敏次、藤井　穂高：ゲノム編集技術の応用：enChIP法とその創薬への応用　DOJIN BIOSCIENCE 29「医療応用を目指すゲノム編集」（化学同人）　編集：真下　知士、金田　安史　(2018) 53-64.
• Fujita T, and Fujii H: New directions for epigenetics: application of engineered DNA-binding molecules to locus-specific epigenetic research. Handbook of Epigenetics, Second Edition, Academic Press (Elsevier), Editor: Trygve O. Tollefsbol, (2017) 635-652.
• 藤井　穂高：遺伝子座特異的クロマチン免疫沈降法を用いたエピジェネティック作動薬・抗感染症薬の開発 - バイオベンチャー「Epigeneron」の取り組み　実験医学増刊「All About ゲノム編集」（羊土社）　編集：真下　知士、山本　卓　(2016) 34 (20): 185-191.
• 藤田　敏次、藤井　穂高：CRISPR/Cas9を用いたenChIP法による遺伝子座特異的ゲノム機能解析　「進化するゲノム編集技術」（株式会社エヌ・ティー・エス）　(2015) 第1編　第2章　第3節
• Fujita T, and Fujii H: Isolation of specific genomic regions and identification of associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Methods Mol. Biol. (2015) 1288:43-52. doi: 10.1007/978-1-4939-2474-5_4.
  PubMed
• 藤井　穂高: TALやCRISPRを用いたenChIP法による特定ゲノム領域の単離と結合分子の同定. 実験医学別冊「今すぐ始めるゲノム編集」（羊土社）　編集：山本　卓(2014) 42-43
• 藤井　穂高: iChIP法による特定ゲノム領域の単離と結合分子の同定. 遺伝子医学Mook「エピジェネティクスと病気」（メディカルドゥ社）監修：佐々木　裕之、編集：中尾　光善、中島　欽一　(2013) 25:254-259

特許

• 登録番号： 特許第6928323号（国内）、特許第6964900号（国内）
  発明者： 藤井　穂高、藤田　敏次、谷川　直紀
  発明の名称： 核酸増幅の特異的抑制方法
  特許権者： 国立大学法人大阪大学
  登録日： 2021 年 8 月 11 日（国内）、2021年10月22日（国内）
• 公開番号： WO 2021/033720
  登録番号： 特許第6765696号（国内）
  発明者： 藤井　穂高
  発明の名称： 学術文献に記載された技術の新規性の判断を支援するための方法、プログラム、およびシステム
  特許権者： 国立大学法人弘前大学
  登録日： 2020 年 9 月 18 日（国内）
• 公開番号： WO 2019/203350
  登録番号： 特許第6653932号（国内）、CA 3096462（カナダ）、KR 10-2285085（韓国）、IL 277764（イスラエル）、US 11155873（米国）、SG 11202009878V（シンガポール）
  発明者： 藤井　穂高、藤田　敏次
  発明の名称： 標的核酸領域内の基準配列に対する差異を検出する方法 / Method for detecting difference in reference sequence in target nucleic acid region
  特許権者： 合同会社Wisteriagen
  登録日： 2020年1月31日（国内）、2021年7月27日（カナダ）、2021年7月28日（韓国）、2021年10月1日（イスラエル）、2021年10月26日（米国）、2022年9月16日（シンガポール）
• 登録番号： 特許第5954808号（国内）、EP 2963113（欧州）、US 11466306（米国）
  発明者： 藤井　穂高、藤田　敏次
  発明の名称： 内在性DNA配列特異的結合分子を用いる特定ゲノム領域の単離方法 / Method for isolating specific genomic region using molecule binding specifically to endogenous DNA sequence
  特許権者： 国立大学法人大阪大学
  登録日： 2016 年 6 月 24 日（国内）、2019 年 11 月 6 日（欧州）、2022年10月11日（米国）
• 登録番号： US 8415098（米国）、特許第5413924号（国内）
  発明者： 藤井　穂高、藤田　敏次
  発明の名称： 特定ゲノム領域の単離方法 / Method for isolating specific genomic regions
  特許権者： 国立大学法人大阪大学
  登録日： 2013 年 4 月 9 日（米国）US8415098、2013 年 11 月 22 日（国内）
• 登録番号： US 7435546
  発明者： Fujii, Hodaka (New York, NY)
  発明の名称： System to detect inducible translocation
  特許権者： New York University
  登録日： 2008 年 10 月 14 日（米国） US7435546